Abigail (Abby) Buchwalter, Ph.D.

Positions and Education

University of California, San Francisco, 10/2018-
Assistant Professor in Residence
Cardiovascular Research Institute
Department of Physiology

The Salk Institute for Biological Studies, 2011-2018
Postdoctoral Fellow with Dr. Martin W. Hetzer
Disruption of the nuclear lamina in premature aging (2014-2018)
Transcription-dependent dynamics of mobile nucleoporins (2011-2014)

Marine Biological Laboratory, 2011
Participant, Physiology Course

Washington University in St. Louis, 2005-2011
Graduate Researcher with Dr. Phyllis I. Hanson
Targeting and activity of the AAA+ ATPase TorsinA within the endoplasmic reticulum
Ph.D. in Biochemistry
Olin Dissertation Award

Hope College, 2001-2005
B.S. in Chemistry with Biochemistry Emphasis
Minor in Mathematics
cum laude

 

Publications

Research Articles

Buchwalter A, Hetzer MW. Nucleolar expansion and elevated protein translation in premature aging. Nature Communications (2017) 8:328.

Goodchild RE, Buchwalter A, Naismith TV, Holbrook K, Billion K, Dauer WT, Liang CC, Dear ML, Hanson PI. Access of torsinA to the inner nuclear membrane is activity dependent and regulated in the endoplasmic reticulum. J Cell Sci (2015) 128(15): 2854-65.

Buchwalter A, Liang Y, Hetzer MW. Nup50 is required for cell differentiation and exhibits transcription-dependent dynamics. Mol Biol Cell (2014) 25(16): 2472-84.

Vander Heyden AB, Naismith TV, Snapp EL, Hanson PI. Static retention of the lumenal monotopic membrane protein torsinA in the endoplasmic reticulum. EMBO J (2011) 30(16): 3217-31.

Vander Heyden AB, Naismith TV, Snapp EL, Hodzic D, Hanson PI. LULL1 retargets TorA to the nuclear envelope revealing an activity that is impaired by the DYT1 dystonia mutation. Mol Biol Cell (2009) 20(11): 2661-72.

Buchwalter A, Van Dort C, Schultz S, Smith R, Le IP, Abbott JL, Oosterhouse E, Johnson AE, Hansen-Smith F, Burnatowska-Hledin M. Expression of VACM-1/cul-5 mutant in endothelial cells induces MAPK phosphorylation and maspin degradation and converts cells to the angiogenic phenotype. Microvasc Res (2008) 75 (2): 155-168.

Johnson AE, Le IP, Buchwalter A, Burnatowska-Hledin MA. Estrogen-dependent growth and estrogen receptor (ER)-alpha concentration in T47D breast cancer cells are inhibited by VACM-1, a cul-5 gene. Mol Cell Biochem (2007) 301 (1-2): 13-20.

Drenan RM, Doupnik CA, Jayaraman M, Buchwalter AL, Kaltenbronn KM, Huettner JE, Linder ME, Blumer KJ. R7BP augments the function of RGS7-Gb5 complexes by a plasma membrane-targeting mechanism. J Biol Chem (2006) 281: 28222-28231.

Review Articles

Buchwalter A, Kaneshiro J, Hetzer MW. Coaching from the sidelines: the nuclear periphery in genome organization. In press, Nature Reviews Genetics (2018).

Buchwalter AB, Hetzer MW. Nuclear pores set the speed limit for mitosis (preview). Cell (2014).

 

Grant Support – Active

Chan Zuckerberg Biohub Investigator, 2018-2023

 

Grant Support – Completed

Ruth L. Kirschstein National Research Service Award Postdoctoral Fellowship, 2012-2015
Identifying links between the nuclear pore complex and the cell cycle (F32GM103084, NIGMS)

Ruth L. Kirschstein National Research Service Award Predoctoral Fellowship, 2009-2011
Mechanism and regulation of LULL1-directed NE enrichment of TorsinA (F31NS065608, NINDS)

 

Honors

 Research Awards

Best Postdoctoral Presentation, Salk/UCSD Biology Program Retreat, 2015
Young Investigator Award, Alternative Muscle Club Meeting, 2015
Spencer T. and Ann W. Olin Fellow (Dissertation Award), 2011
Sigma Xi Undergraduate Research Award in Chemistry, 2005
Undergraduate Travel Award, Association for Biochemistry and Molecular Biology Meeting, 2004

Teaching and Outreach

Stavros Niarchos Foundation New Frontiers Salk Researcher Specialist Award, 2016
American Society for Cell Biology COMPASS Outreach Grant Award, 2014

 Scholarships

Jaecker Scholarship in Chemistry, 2004-2005
Hope College Presidential Scholarship, 2001-2005

 

Outreach

The Salk Institute for Biological Studies

Outreach: Co-founded and secured initial funding (ASCB COMPASS grant) for the SciChats @ Salk science outreach program (http://education.salk.edu). Video-chat-based science discussions between Salk scientists and elementary school students, 2014-2016

 Washington University in St. Louis

Outreach: Led Chemistry Teaching Team, Young Scientist Program. Washington University science education outreach organization (http://ysp.wustl.edu/). Organized graduate student volunteers, developed and taught chemistry experiment demonstrations at St. Louis area high schools, 2005-2010

 

Selected Presentations

Research

Protein turnover at the nuclear periphery: insights and surprises. Talk. Nuclear Organization Meeting, Cold Spring Harbor Laboratory, May 2018.

Lamin A regulates the activity and dynamics of nucleoli. Talk. American Society for Cell Biology Meeting, December 2017.

The aging nucleus: from declining nuclear function to disrupted cell metabolism. Invited speaker. Cell Biology and Physiology seminar series, Washington University in St. Louis, October 2017.

Nucleolar expansion in premature and physiological aging. Talk. Emerging Functions of the Nucleolus ASBMB Symposium, October 2017.

The aging nucleus: from declining nuclear function to disrupted cell metabolism. Talk. Salk Featured Fellows Series, October 2017.

Elevated nucleolar activity in premature and physiological aging. Talk. La Jolla Aging Meeting, April 2017.

Mutant lamin A accelerates protein synthesis in premature aging. Talk. American Society for Cell Biology Meeting, December 2016.

Living fast: accelerated protein synthesis in premature aging. Talk. Salk Science at the Seaside Retreat, October 2016.

Protein turnover at the nuclear periphery. Talk. American Society for Cell Biology Meeting, December 2015.

Protein turnover at the nuclear periphery. Talk. The Nuclear Lamina in Health and Disease Workshop, November 2015.

Protein homeostasis at the nuclear periphery, a mutation hotspot for muscular dystrophy. Talk. Alternative Muscle Club Meeting, September 2015.

A link between the mobility of the nucleoporin Nup50 and the transcriptional state of chromatin. Poster. American Society for Cell Biology Meeting, December 2013.

Peripheral membrane association controls the subcellular targeting of the luminal enzyme TorsinA. Talk. American Society for Cell Biology Meeting, December 2010.

A novel mechanism underlying ER retention of the luminal enzyme TorsinA. Poster. Keystone Symposium, January 2010.

LULL1 controls the targeting of TorsinA. Talk. Dystonia Medical Research Foundation Young Investigators Meeting, September 2009.

LULL1 retargets TorA to the nuclear envelope revealing an activity that is impaired by the DYT1 dystonia mutation. Poster. American Society for Cell Biology Meeting, December 2008.

VACM-1/cul-5 regulates proliferation and angiogenesis by modulating MAPK phosphorylation and maspin protein expression. Poster. (Travel Award), American Society for Biochemistry and Molecular Biology Meeting, 2004.

Education

SciChats Live: A family-friendly virtual lab tour. Talk. Explore Salk Community Science Day. April 2015 and 2016.

SciChats @ Salk connects students and scientists for informal video chat conversations. Talk. American Society for Cell Biology Meeting December 2014.

The Young Scientist Program at Washington University in St. Louis. Talk. American Society for Cell Biology Meeting December 2008.